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Self-limiting aggregation of phospholipid vesicles

Self-limiting aggregation of phospholipid vesicles† N. de Lange, F. A. M. Leermakers and J. M. Kleijn * Lipid vesicles are widely used as model systems to study biological membranes. The self-assembly of such vesicles into vesicle pairs provides further opportunity to study interactions between membranes.

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Leakage and aggregation of phospholipid …

Vesicles comprising 30% cholesterol, 20% DOPG and 50% DOPC also caused a clear drop in the induction efficiency of BID ( Fig. 4). These vesicle leakage results resemble those obtained using the pore‐forming peptide synthetic GALA, which is similarly affected by DOPE and cholesterol [[27, 28]].

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Aggregation and fusion of phospholipid vesicles …

Membrane fusion and aggregation of phospholipid vesicles are reviewed and discussed. The fusion process is viewed as consisting of several stages: aggregation and close apposition of the particles, destabilization, and finally, merging of the bilayers. A procedure is presented which yields both the rate constant of the dimerization (C 11 >) and the rate constant for fusion of the dimers (f ...

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Preparation of block copolymer vesicles in …

Block copolymer vesicles can be prepared in solution from a variety of different amphiphilic systems. Polystyrene‐block ‐poly(acrylic acid), polystyrene‐block ‐poly(ethylene oxide), and many other block copolymer systems can produce vesicles of a wide range of sizes; those in the range of 100–1000 nm have been explored extensively.. Different factors, such as the absolute and ...

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(104f) Directed Aggregation and Fusion of …

Higher order or secondary levels of self-assembly of lipid vesicles have been achieved through vesicle aggregation using electrostatic and ligand–receptor interactions. These higher ordered assemblies of lipid vesicles were also studied as a simple model for biological tissues.

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Aggregation of Cyanine Dye in Bilayer Vesicles of ...

Aggregation of Cyanine Dye in Bilayer Vesicles of Phospholipids Casey McCullough, Matthew Heywood, and Hussein Samha Division of Chemistry Southern Utah University 351 W. Center Street, SC 215 Cedar City, Utah 84720 USA Received: April 1, 2005 Accepted: September 26, 2005 ABSTRACT

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IJMS | Free Full-Text | Lipid Vesicle Aggregation …

Lipid bilayer fusion is a complex process requiring several intermediate steps. Initially, the two bilayers are brought into close contact following removal of intervening water layers and overcoming electrostatic repulsions between opposing bilayer head groups. In this study we monitor by light scattering the reversible aggregation of phosphatidylcholine single shell vesicles during which ...

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Cholesterol catalyses Aβ42 aggregation through …

Habchi, J., Chia, S., Galvagnion, C. et al. Cholesterol catalyses Aβ42 aggregation through a heterogeneous nucleation pathway in the presence of lipid …

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Self-limiting aggregation of phospholipid …

Lipid vesicles are widely used as model systems to study biological membranes. The self-assembly of such vesicles into vesicle pairs provides further opportunity to study interactions between membranes. However, formation of vesicle pairs, while subsequently keeping their colloidal stability intact, is chall

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Critical molecular weight effects in the …

The surface modification of vesicles with glycolipids (O 1,O 5-bis(octadecyl)N-maltooligonoyl-L-glutamate) accelerates the aggregation of vesicles induced by dextran; therefore, M c significantly decreased due to the surface modification.

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Isolation of Extracellular Vesicles: General …

Background . Extracellular vesicles (EVs) play an essential role in the communication between cells and transport of diagnostically significant molecules. A wide diversity of approaches utilizing different biochemical properties of EVs and a lack of accepted protocols make data interpretation very challenging. Scope of Review . This review consolidates the data on the classical and state-of ...

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High-speed centrifugation induces aggregation …

Keywords: extracellular vesicles, blood plasma, cryo-electron microscopy, immuno-gold electron microscopy, flow cytometry, vesicle aggregation, centrifugation Blood and other body fluids contain membrane vesicles that are released by cells upon activation or apoptosis ( 1 – 3 ).

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Aggregation of phospholipid vesicles by water …

Water-soluble polymers such as dextran and polyethylene glycol are known to induce aggregation and size growth of phospholipid vesicles. The present study addresses the dependence of these processes on vesicle size and concentration, polymer molecular weight, temperature, and compartmentalization of the vesicles and polymers, using static and dynamic light scattering.

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Alpha-synuclein - Wikipedia

Alpha-synuclein is a protein that, in humans, is encoded by the SNCA gene. It is abundant in the brain, while smaller amounts are found in the heart, muscle and other tissues. In the brain, alpha-synuclein is found mainly at the tips of neurons in specialized structures called presynaptic terminals. Within these structures, alpha-synuclein interacts with phospholipids and proteins.

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Self-limiting aggregation of phospholipid …

Request PDF | Self-limiting aggregation of phospholipid vesicles | Lipid vesicles are widely used as model systems to study biological membranes. Self-assembly of such vesicles into vesicle pairs ...

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Aggregation of Plant Protoplasts by Artificial …

Sonicated unilamellar lipid vesicles, consisting of egg lecithin, stearylamine, and cholesterol in 7:2:1 molar ratios, promoted the aggregation of tobacco ( Nicotiana glutinosa ) protoplasts with the aid of mono- or divalent cations. A reaction mixture containing liposomes (0.4 micromoles lipid per milliliter), 50 millimolar CaCl2, 0.5 molar mannitol, and 5 × 105 protoplasts per milliliter ...

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Aggregation of Amphiphiles as Micelles or Vesicles in ...

Aggregation of Amphiphiles as Micelles or Vesicles in Aqueous Media R. NAGARAJAN AND E. RUCKENSTEIN State University of New York at Buffalo, Faculty of Engineering and Applied Sciences, Buffalo, New York, 14214

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Glucocerebrosidase deficiency promotes protein …

Author summary Mutations in the GBA gene, which encodes the enzyme glucocerebrosidase, are common and increase the risk of Parkinson disease. A widely accepted explanation for the increased risk is that the fatty substance normally broken down by glucocerebrosidase builds up in the lysosome, which is the cell's recycling center, until the cell can no longer get rid of damaged parts.

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Aggregation of phospholipid vesicles by water …

Water-soluble polymers such as dextran and polyethylene glycol are known to induce aggregation and size growth of phospholipid vesicles. The present study addresses the dependence of these processes on vesicle size and concentration, polymer molecular weight, temperature, and compartmentalization of the vesicles and polymers, using static and dynamic light scattering.

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Key role of the N‐terminus of chicken annexin A5 …

Abstract Annexins are calcium‐dependent phospholipid‐binding proteins involved in calcium signaling and intracellular membrane trafficking among other functions. Vesicle aggregation is a crucial ev...

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